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Scientific background |
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Fluorescence is the emission of a photon by a molecule following its excitation by an electromagnetic radiation of a precise wavelength. This excitation induces an energy jump from a fundamental electronic state S0 to an upper electronic energy level S1. The molecule comes back to its fundamental energy level while a photon is emitted. The photon energy corresponds to the energy difference between the two levels S0 and S1. Some small energy losses are due to internal conversion among the different vibrational and rotational levels of each electronic state of the molecule. |
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That's why E1 (excitation energy) is always greater than E2 (emission energy). |
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