Scientific background

LIF Labeling agent

FAQ

 

Common fluorescent tags for derivatization are listed in the table below :

For amine function (amines, amino acids, peptides, proteins…)

Primary and secondary amine functions :

Fluorescein isothiocyante (FITC)

488 nm

 

4-fluoro-7-nitrobenz-2-oxa-1,3-diazole (NBD-F)

488 nm

 

Dansyl chloride 325 nm

325 nm

 

 

 

Primary amine function :

naphtalene 2,3- dicarboxaldehyde (NDA)

442 nm

 

3-(4-carboxybenzoyl)-quinoline-2-carboxaldehyde (CBQCA)

442 nm

 

fluorescamine

354 nm

 

orthophthaldialdehyde (OPA)

325 nm

 

 

 

For hydroxy function

alcohol

Fluorescein-5-carbonylazyde

488 nm

 

 

 

sugars

9-aminopyrene-1,4,6 trisulfonate (APTS)

488 nm

 

9-aminonaphtalene-1,4,6 trisulfonate (ANTS)

354 nm

 

fluorescamine

354 nm

 

amino benzamide

325 nm

 

 

 

 

For thiol function (amino acids, peptides, proteins…)

 

5-iodo-acetamido-fluorescein (IAF)

488 nm

 

4-fluoro-7-nitrobenz-2-oxa-1,3-diazole (NBD-F)

488 nm


For aldehyde and ketone functions

 

fluorescein-5-thiosemicarbazide

488 nm

 

For carboxylic function

 

5-bromomethyl fluorescein

488 nm

 

 

More about derivatization :

  • Lunn G., Hellwig L.C. " Handbook of derivatization reactions for HPLC " Wiley Interscience, New York, 1998. (ISBN0-471-23888-0)
  • Toshimasa Toyo'oka " Modern derivatization methods for separation sciences " Wiley Interscience, New York, 1999. (ISBN0-471-98364-0)
  • Bayle C., Couderc F. "Fluor-up : Updated references on derivatization reactions for HPLC and CE " , Picometrics Data Base, Picometrics, 2000.
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